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KMID : 0545120000100010111
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 1 p.111 ~ p.114
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Characterization of Benzoate Begradation via ortho-Cleavage by Streptomyces setonii
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AN, HAE-REUN
PARK, HYUN-JOO/KIM, EUNG-SOO
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Abstract
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Streptomyces are widespread in nature and play a very important role in the biosynthesis as well as biodegradation of natural and unnatural aromatic compounds. Both qualitatively and quantitatively through TLC and UV spectrophotometric assays, it was observed that the thermophilic soil bacteria S. setonii (ATCC 39116), which can utilize a benzoate as a sole carbon and energy source in a minimal liquid culture, was not very sensitive to the benzoate concentration and to the culture conditions such as the pH and temperature. The in vitro conversion of a catechol to a cis,cismuconic acid by a crude S. setonii lysate implies that the aromatic ring cleavage by S. setonii is initiated by a thermostable catechol-1,2-dioxygenase, the key enzyme in the ortho-cleavage pathway of aromatic compound biodegradation. Unlike nondegrading S. lividans, S. setonii was also highly resistant to other similar hazardous aromatic compounds, exhibiting almost no adverse effect on its growth in a complex liquid culture.
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